Blog20Artikel
Notizen zu Probenaufgabe und Injektion.
Praxisleitfäden zu HPLC-Autosamplern, Injektion, Laborautomatisierung und Gerätebau, mit Quellenangaben. Vom Team, das den Elutrolab AS-48 entwickelt.
ENDie Artikel sind auf Englisch.
Zum Einstieg
Top 3 compact HPLC autosamplers in 2026 (and how to choose one)
Three compact HPLC autosamplers you can buy in 2026, compared on verified size, vials, pressure and control, plus a checklist for choosing one.
Vergleiche1
HPLC dimensions and bench space: module footprints compared
HPLC dimensions from current datasheets: autosampler and system footprints, service clearances, bench depth, lab space cost and practical ways to save room.
8 Min. Lesezeit
Leitfäden9
How an HPLC autosampler works: injection principles explained
How an HPLC autosampler works, step by step: needle, syringe, loop, valve and wash, plus fixed-loop, flow-through needle and split-loop designs compared.
10 Min. Lesezeit
Full-loop vs partial-loop injection (and how to size a sample loop)
Full-loop vs partial-loop injection compared: precision, overfill, the 50% rule, syringe accuracy and sample use, with worked examples for sizing a sample loop.
9 Min. Lesezeit
HPLC injection volume: how much can you inject on your column?
How much can you inject on an HPLC column? Column volume formula, a table of maximum injection volumes for common columns, and how diluent strength changes it.
8 Min. Lesezeit
Autosampler carryover: causes, how to measure it and how to cut it
How to calculate autosampler carryover, what ICH M10 accepts, how to find the part that causes it, and which wash solvents and rinse settings reduce it.
11 Min. Lesezeit
HPLC pressure ratings: psi to bar, MPa and what your autosampler needs
HPLC pressure rating explained: psi to bar and MPa conversions, a column backpressure calculation, viscosity and temperature effects, and how to size an autosampler.
11 Min. Lesezeit
HPLC vials, caps and septa: a practical selection guide
How to choose HPLC vials, caps and septa: neck finishes, glass or polypropylene, inserts with real residual volumes, septum trade-offs and worked examples.
11 Min. Lesezeit
Autosampler qualification: the tests to run on a new autosampler
HPLC autosampler qualification explained: USP <1058> status in 2026, the OQ tests with typical acceptance limits, and worked precision and gravimetric examples.
10 Min. Lesezeit
Online HPLC reaction monitoring: how automated reactor sampling works
Online HPLC reaction monitoring explained: offline vs at-line vs online, reactor sampling, quench and dilution, published systems and a worked sampling schedule.
10 Min. Lesezeit
Buying a used or refurbished HPLC autosampler: prices and checklist
Used and refurbished HPLC autosampler prices seen in October 2026, what each grade includes, a pre-purchase checklist and a five-year cost of ownership example.
10 Min. Lesezeit
Technik4
6-port injection valve explained: positions, rotor seals and pressure
How a 6-port HPLC injection valve works: load and inject positions port by port, rotor seal materials and pH limits, pressure ratings, wear and maintenance.
10 Min. Lesezeit
Controlling serial lab instruments from Python with pyserial
A practical pyserial guide for lab instruments: finding ports, timeouts, line endings, a tested driver class, retries, asyncio, fake devices and OS quirks.
7 Min. Lesezeit
SiLA 2, OPC UA LADS and serial: lab instrument integration standards
SiLA 2, OPC UA LADS, SCPI and plain serial protocols compared on transport, discovery, typing, tooling and effort, with a tested SiLA 2 wrapper for a serial pump.
9 Min. Lesezeit
OEM autosampler checklist: building one into your instrument
An OEM autosampler checklist for analyzer developers: mechanics, fluidics, 24 V power, serial protocol, IEC 61010 and CE compliance, lifecycle, supplier questions.
11 Min. Lesezeit
Forschung3
The future of HPLC autosamplers: trends for the next decade
Eight autosampler trends for the next decade, from capillary LC and SiLA 2 to self-driving labs and online PAT, split into what is real now and what is not.
10 Min. Lesezeit
Self-driving labs: where sampling and HPLC injection fit in the loop
What a self-driving lab is, how landmark systems analysed their samples, why HPLC is often the bottleneck, and a checklist for samplers that run in a closed loop.
8 Min. Lesezeit
Open-source and low-cost autosamplers: what the literature shows
A review of 14 published open-source and DIY autosamplers and sampling robots: what they do, what they cost, and why almost none inject at HPLC pressure.
11 Min. Lesezeit
Nachschlagen2
HPLC autosampler troubleshooting: common problems and fixes
Autosampler troubleshooting by symptom: poor precision, missing and ghost peaks, pressure spikes, leaks, blocked needles and split early peaks, with tests and fixes.
19 Min. Lesezeit
HPLC autosampler glossary: 72 terms explained
An HPLC autosampler glossary: 72 terms from backflush and carryover to split-loop, SiLA 2 and virtual COM port, each with a short definition and typical values.
15 Min. Lesezeit